Fig. 3.

Immunocytochemical staining of the primary Kupffer cells, KUP5, MG6 and BMDM cell plated on eight-well chamber glass slides in DMEM-based medium containing 10% FCS. The cells were fixed and stained with specific antibodies against Mac-1, F4/80, and rat IgG, as described in Section 2. Mac-1 and F4/80 are cell surface markers, but the antibody–antigen reaction was so intense that the cellular and nuclear structures were difficult to distinguish under a phase contrast microscope. A negative control, rat IgG showed only faint immunostaining. Multinucleated giant cells were occasionally observed in the culture of KUP5 cells (arrowheads). Scale bar = 100 µm.