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. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: Glia. 2013 Oct 28;62(1):17–25. doi: 10.1002/glia.22580

FIGURE 1.

FIGURE 1

High-fat diet (HFD) increases the total number of microglia and the number of activated iba1-positive microglia in the arcuate nucleus of the hypothalamus. Wild-type mice fed a standard chow (SC) diet have a modest number of microglia (expressing eGFP (green) under the control of the endogenous Cx3cr1 locus) (B), including some iba1-positive cells (red, arrows in A and C). After 6 weeks of HFD diet, both the total number of microglia (E) and the number of iba1-positive microglia (D) are increased. Under these conditions, only a proportion of the total microglial population is still iba1-positive (F). III indicates third ventricle; scale bar = 25 μm.