Table 2.

Experimental design—RSM for media optimisation.

Run order	Point type	Block	BHB	BE	Glycerol	SF oil	E1 average
1	1	1	1	3	4	4	38
2	0	1	2	2	3	3	42.31
3	1	1	1	1	2	4	50
4	−1	1	2	2	2	3	37
5	1	1	1	1	4	4	46.15
6	1	1	3	1	2	4	34.61
7	1	1	3	1	4	4	40.44
8	1	1	3	3	4	2	35.71
9	1	1	1	3	2	4	41.4
10	0	1	2	2	3	3	40
11	−1	1	2	3	3	3	36
12	1	1	1	3	4	2	40
13	0	1	2	2	3	3	42.31
14	1	1	1	3	2	2	46
15	−1	1	2	2	4	3	36
16	−1	1	2	2	3	4	40
17	−1	1	3	2	3	3	36
18	−1	1	1	2	3	3	42.9
19	1	1	3	3	2	4	33.33
20	1	1	1	1	2	2	52
21	0	1	2	2	3	3	40
22	0	1	2	2	3	3	40
23	0	1	2	2	3	3	40
24	1	1	3	1	4	2	44.44
25	1	1	1	1	4	2	46
26	−1	1	2	2	3	2	46.15
27	1	1	3	3	4	4	28
28	1	1	3	3	2	2	37.33
29	0	1	2	2	3	3	40
30	−1	1	2	1	3	3	41.94
31	1	1	3	1	2	2	44.44