Table 2. Blood Biomarkers and Dietary Intake by B12 Status Group.
| Serum B12 Groups |
||||
|---|---|---|---|---|
| All | Hi-B12 | RR-B12 | P-value1 | |
| Blood Biomarker | ||||
| B12, pg/mLA | 1083 (371, 3455) | 1414 (823, 3455) | 853 (371, 1284) | <0.001 |
| B6, ng/mL2,A | 16.9 (5.0, 91.7) | 17.6 (5.0, 91.7) | 15.4 (5.4, 39.4) | 0.05 |
| RBC folate, ng/mL3,A | 496 (231, 1045) | 509 (231, 857) | 488 (278, 1045) | 0.95 |
| Homocysteine, μmol/LA | 4.8 (2.2, 9.0) | 4.7 (2.2, 8.9) | 5.1 (2.8, 9.0) | 0.01 |
| Methionine, nmol/mLA | 24.9 (11.1, 65.7) | 23.5 (16.1, 65.7) | 25.9 (11.1, 56.3) | 0.46 |
| Cysteine, nmol/mL | 43.7 (12.4, 79.0) | 46.5 (12.4, 66.3) | 41.4 (28, 79) | 0.01 |
| hsCRP, mg/L 4 | 0.4 (0.1, 48.4) | 0.4 (0.1, 48.4) | 0.4 (0.1, 5.5) | 0.61 |
| WBC, thou/μL 4 | 7.1 (4.1, 17.8) | 7.7 (4.5, 17.8) | 6.9 (4.1, 16.7) | 0.24 |
| Dietary Intake | ||||
| Number | 95 | 54 | 41 | |
| Energy, kcal | 2325 (865, 4909) | 2359 (865, 4909) | 2320 (1413, 4014) | 0.94 |
| %EER | 115 (41, 228) | 114 (41, 223) | 117 (64, 228) | 0.49 |
| Total B12, μg | 18.4 (5.5,158.9) | 21.9 (8.0, 158.9) | 15.1 (5.3, 33.3) | <0.001 |
| Total B12, %RDA | 1175 (310, 8826) | 1391 (479, 8826) | 1000 (310, 1872) | <0.001 |
| Diet , μg | 6.0 (1.5, 18.1) | 6.4 (2.6, 18.1) | 5.6 (1.5, 14.0) | 0.17 |
| Diet, %RDA | 376 (79, 1085) | 379 (79, 1085) | 373 (83, 780) | 0.80 |
| Supplement, %RDA | 667 ( 0, 8333) | 1000 (250, 8333) | 583 (0,1375) | <0.001 |
| 0 - 499 %RDA | 15 | 11 | 20 | |
| 500 - 999 %RDA | 47 | 35 | 63 | |
| 1000 - 1499 RDA | 29 | 39 | 17 | |
| 1500+ %RDA | 8 | 15 | 0 | 0.002 |
| Total B6, mg | 4.6 (1.9, 78.2) | 5.5 (1.9, 78.3) | 3.9 (2.4, 8.3) | <0.001 |
| Total B6, %RDA | 545 (243, 7827) | 591 (243, 7827) | 502 (247, 1131) | 0.01 |
| Diet, mg | 1.9 (0.5, 8.8) | 1.9 (0.7, 8.8) | 1.8 (0.5, 5.6) | 0.13 |
| Diet, %RDA | 217 (51, 931) | 219 (59, 881) | 214 (51, 931) | 0.50 |
| Supplement, mg/d | 2.3 (0.0, 75.0) | 3.0 (0.6, 75.0) | 1.9 (0.0, 4.0) | 0.01 |
| Supplement, %RDA | 317 (0, 7500) | 317 (105, 7500) | 300 (0, 425) | 0.09 |
| Total Folate, μg | 755 (335, 1872) | 933 (376, 1872) | 616 (335, 1518) | <0.001 |
| Total Folate, %RDA | 281 (113, 840) | 332 (128, 840) | 227 (113, 506) | 0.003 |
| Diet, μg | 413 (140, 1472) | 451 (156, 1473) | 385 (140, 1251) | 0.03 |
| Diet, %RDA | 159 (39, 491) | 167 (39, 491) | 143 (47, 417) | 0.14 |
| Supplement, μg | 400 (0, 1200) | 400 (66, 1200) | 200 (0, 800) | 0.004 |
| Supplement, %RDA | 100 (0, 400) | 133 (33, 400) | 100 (0, 267) | 0.02 |
hsCRP = high sensitivity C-reactive protein (<3 mg/L normal); WBC, white blood cell count; %EER, percent Estimated Energy Requirement; RDA = Recommended Dietary Allowance
Means ± SD and t-tests for normally distributed variables; median (range) and Mann-Whitney for non-normally distributed variables.
n=103 (n=58 for Hi-B12, n=45 for RR - B12).
n=97(n=54 for Hi-B12, n=43 for RR - B12).
n=102 (n=57 for Hi-B12, n=45 for RR - B12).
Biomarker Reference Ranges: B12, pg/mL: Males: 4-6.9 years: 245-1078; 7-9.9 years 271-1170; 10-12.9 years 183-1088; 13-18 years 214-865; >18 year old 199-732. Females: 4-6.9 years 313-1407; 7-9.9 years 247-1174; 10-12.9 years 197-1019; 13-18 years 182-820; >18 year old 199-732. (CHOP Clinical Laboratory, Philadelphia, PA). B6, ng/mL: 5-30 (ARUP Laboratories, Salt Lake City, UT). Folate, ng/mL: 280-903 ng/mL (ARUP Laboratories, Salt Lake City, UT). Homocysteine , μmol/L: 6-10.9 years: 0.8-6.5. 11-16.9 years 5.7-11.7. >17 years 10.5-16.7 (ARUP Laboratories, Salt Lake City, UT). Methionine, nmol/mL: 8-49 nmol/mL (ARUP Laboratories, Salt Lake City, UT).