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. 1977 Feb;15(2):608–613. doi: 10.1128/iai.15.2.608-613.1977

Extracellular antigens of Micropolyspora faeni grown in synthetic medium.

V P Kurup, J N Fink
PMCID: PMC421410  PMID: 66202

Abstract

Extracellular antigens were prepared by growing Micropolyspora faeni in a new synthetic medium (SMSL), and this antigenic preparation was compared with antigens obtained by the double-dialysis method. These two preparations were tested against pooled sera from farmer's lung patients and rabbits immunized with these antigens by the agar-gel double-diffusion, immunoelectrophoresis, and two-dimensional immunoelectrophoresis methods. The immunological cross-reactivity between M. faeni strains of diverse origin and between other species of thermophilic actinomycetes associated with hypersensitivity pneumonitis was also investigated. The usefulness of two-dimensional immunoelectrophoresis in the comparison and standardization of antigens is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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