Table 1.

Random mutagenesis approach to the stability of PAI-1.

Mutation	Stability increase	t 1/2	Reference
I91L	9-fold	18 h	[61]
N150H, K154T, Q319L, and M354I	72-fold	145 h	[125]
N150H, K154T, Q319L, M354I, and Q301P	75-fold	150 h	[126]
Combination of mutations at positions 50, 56, 61, 70, 94, 150, 222, 223, 264, and 331	122-fold	245 h	[127]
Disulfide mutant	350-fold	700 h	[128]