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. 2014 Oct 30;6:296. doi: 10.3389/fnagi.2014.00296

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Copyright © 2014 Tamaki, Hirata and Uchiyama.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of fiber-type components in PLT muscle between adult and middle-aged rats. Whole PLT sections were stained for ATPase (preincubation, 4.6); thus, the darkest staining represents Type I, medium Type IIb, and light Type IIa (C). This is likely to give the impression that the distribution of Type I fibers is slightly higher in middle-aged rats (B) than in adult rats (A). Differences were significant when calculated per unit area (section) for Type I and Type IIb (D), thus confirming the histochemical changes from fast- to slow-type fibers. Bars in (A,B) = 1 mm, (C) = 100 μm. ^*P < 0.05.