Figure 4. Localization of 8-OH-dG in the seminal vesicles and ventral prostate of 6 and 26 month Lobund-Wistar rats consuming de-ionized water or green tea.
Eight micron paraffin sections of the seminal vesicles and ventral prostate were prepared from 6 and 26-month-old animals consuming de-ionized water or 0.6% green tea extract. The sections were incubated with antibodies specific for 8-OH-dG as described in Materials and Methods. Panels A and B highlight weak and intense staining for 8-OH-dG respectively in nuclear regions of the epithelium in the seminal vesicles in 6 and 26-month-old animals. Panels C and D highlight weak and intense staining for 8-OH-dG in nuclear regions of the epithelium in the ventral prostate in 6 and 26-month-old animals. Bar graphs underneath the panels display the intensity of staining for 8-OH-dG in the seminal vesicle and prostate, scoring two cores per animal (n=12) Legend: 3- strong staining, 2- moderate staining, 1- weak staining, 0- no staining.