Figure 1. Sequence and predicted secondary structure of the coligos used in Figure 2. Coligo 122 transcription start site (TSS, arrow) and transcript 3′ end site (Stop) were identified previously by cDNA sequencing.⁸ TSS of coligo 19am3 and 19anbm34 were predicted based on their close similarity to coligo 19aTAR transcript sequencing data.⁸ The TSS for coligos 221, luc-2, luc-1, Dcr3, and 15a-b are predicted to be at a pyrimidine close to the 3′ end of the DNA stem, near its junction with the larger loop. Transcript 3′ end sites, resulting from termination or termination with nuclease trimming, are predicted to be at the 5′ end of the DNA stem, based on previous observations.⁸ Coligo luc-2 and luc-1 differ only by one nucleotide (circled) in the larger terminal loop region. Shaded regions indicate cDNA of mature miRNA in those coligos designed from human genomic miRNA loci. Black triangles denote the 3′ and 5′ ends of the linear precursor deoxyoligonucleotides, where the sequences were ligated to form a circle. Coligo sizes are shown in nucleotides (nt); solid lines above sequences indicate known RNA RNAP III termination sequences.