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Compound 1 inhibits the KIX–KID interaction in vitro. A) Renilla luciferase activity was specifically reconstituted upon mixing RLucC–KIX and KID–RLucN. B) Compound 1 inhibits the KIX–KID interaction in vitro. RLucC–KIX and KID–RLucN were combined in the presence of varying concentrations of 1 (●), 2 (■) or 3 (▲). The mixture was then incubated on ice for 24 h, and the remaining luciferase activity was measured.
