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. 2014 Aug 12;10(5):2358–2364. doi: 10.3892/mmr.2014.2485

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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DSC2 silencing in ESCC cells by siRNAs. (A) RT-qPCR analysis of the expression of DSC2 in ESCC cell lines. The expression levels of DSC2 were normalized to that of β-actin. (B) RT-qPCR analysis of DSC2 silencing by siRNAs. Cells were transfected with DSC2 siRNA or control siRNA. (C) DSC2 silencing in SHEEC and KYSE510 cells was evaluated using western blot analysis. β-actin served as a loading control. (D) Immunofluorescence analysis of DSC2 silencing by siRNAs (magnification, ×400). DSC2, desmocollin-2; ESCC, esophageal squamous cell carcinoma; RT-qPCR, reverse transcription quantitative polymerase chain reaction.