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. 2014 Oct 30;8:366. doi: 10.3389/fnbeh.2014.00366

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Copyright © 2014 Alhaddad, Kim, Aal-Aaboda, Althobaiti, Leighton, Boddu, Wei and Sari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of MS-153 at 50 mg/kg (MS-153/50, n = 5), vehicle (ethanol vehicle group, n = 5), naïve (naïve ethanol vehicle group, n = 5) groups on NFκ B-p65 level in the NAc. (A,C,E) Immunoblots for GAPDH and Lamin [which were used as control loading proteins for cytoplasmic extract (CE) and nuclear extract (NE) proteins, respectively], NFκ B-p65, and Iκ Bα in CE and NE. (B,D) Quantitative analysis of NFκ B-p65 levels in the CE and NE revealed a significant increase in the ratio of NFκ B-p65/Lamin level in the NE but not in the CE for the ethanol MS-153-treated group compared to the naïve ethanol vehicle and ethanol vehicle groups. (F) Quantitative analysis of Iκ Bα level in the CE revealed a significant decrease in the ratio of Iκ Bα/GAPDH level in the ethanol MS-153-treated group compared to the naïve ethanol vehicle and ethanol vehicle groups. Values shown as means ± s.e.m. (^*p < 0.05).