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. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: Trends Cell Mol Biol. 2013;8:13–23.

Figure 4. The intranucleosomal EC+49 formed by T7 RNAP is homogeneous and stable.

Figure 4

A. The experimental approach. The template allows progression and stalling of T7 ECs at −5 and +49 positions (relative to promoter-proximal boundary of the nucleosome) upon addition of different partial combinations of NTPs. B. Homogenous and active EC+49 complex is formed by T7 RNAP. T7 EC+49s were formed on the DNA or nucleosome and then extended in the presence of all NTPs. Analysis of pulse-labeled RNA by denaturing PAGE. Note that the EC+49 is functional and contains RNA, which is extendable upon addition of all NTPs.