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. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: Trends Cell Mol Biol. 2013;8:13–23.

Figure 5. DNA is uncoiled from the octamer behind T7 EC+49.

Figure 5

A. Analysis of the structure of T7 EC+49 formed on DNA or nucleosomal templates (N) by DNase I footprinting. Analysis of end-labeled DNA by denaturing PAGE. A part of the gel is shown at a higher magnification on the left. The footprints of the EC+49 (dotted line), the uncoiled DNA region (dashed line), the position of the active center (EC+49, arrow) and the nucleosome dyad (square) are shown. The suggested structure of the EC+49 complex formed on the 603 nucleosomal template is shown in the inset. Note that DNA behind T7 RNAP in the EC+49 is more accessible to DNase I than in the intact nucleosome, indicating its partial uncoiling from the octamer. B. Quantitative analysis of DNase I footprinting (Fig. 5A). Lanes 3 (603 nucleosome) and 4 (T7 EC+49 in the 603 nucleosome) in Fig. 5A were scanned and scans were aligned pairwise.