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. 2014 Apr 10;25(11):2573–2583. doi: 10.1681/ASN.2013050565

Figure 4.

Figure 4.

Sdccag8 is required for photoreceptor maintenance. (A–C) Histologic analysis of H&E-stained wild-type and Sdccag8gt/gt retinas at P30 (A), P100 (B), and P250 (C) show progressive degeneration of photoreceptor inner segments and outer segments, as well as severe reduction of the outer nuclear layer in Sdccag8gt/gt mice. (D and E) Immunolocalization of CEP164 and rhodopsin in the P30 retina shows rhodopsin accumulation (white arrows) at the photoreceptor inner segment plasma membrane and cell bodies in Sdccag8gt/gt retina (E). (F and G) PNA-FITC lectin staining of cone sheaths at P30 demonstrates a reduction in cone cell number in Sdccag8gt/gt retina (G). H&E, hematoxylin and eosin; RPE, retinal pigment epithelium; ROS, retinal outer segment; IS, inner segment; ONL, outer nuclear layer; INL, inner nuclear layer; ISL, inner plexiform layer; GCL, ganglion cell layer; wt, Sdccag8 wild-type allele; gt, Sdccag8 gene-trap allele; PNA, peanut agglutinin lectin. Bar, 100 μm in A–C; 10 μm in D and E; 50 μm in F and G.