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. 2014 Apr 10;25(11):2499–2510. doi: 10.1681/ASN.2013070786

Figure 2.

Figure 2.

Temporal evaluation of renal injury in FHH and Nr4a1−/− rats at weeks 8, 16, and 24. (A) Glomerular, tubulointerstitial, and vascular injury. Each glomerulus is scored on a scale from 0 (normal) to 4 (global sclerosis). A minimum of 20 glomeruli are scored for each kidney section (n=6 per group/time). For tubulointerstitial injury (fibrosis), a minimum of 20 random images per kidney section are studied (n=6 animals per group). Vascular injury is assessed by measurement of vessel wall thickening. Vessel wall thickening (vessel media, micrometers squared) is calculated by measuring the outer circumference of the vessel minus the inner circumference of the lumen (20 random images per animal, n=6 animals per group). (B) Representative images of kidney sections from FHH and Nr4a1−/− rats at week 24. (C) Quantification of macrophage (ED-1–positive cells) infiltration in glomeruli (weeks 8, 16, and 24) and representative immunohistochemistry images at week 24. (D) Macrophage infiltration in tubulointerstitial region (weeks 8, 16, and 24) and representative immunohistochemistry images at week 24. *P<0.05 compared with FHH.