Figure 2.

Iron sucrose enhanced NF-κB binding activity and increased expression of VCAM-1 and ICAM-1 in HAECs. NF-κB binding activity was determined by assaying nuclear lysates using DNA binding ELISA. Protein levels were determined with Western blotting. (A) Dose-dependent and (B) time-dependent effects of iron sucrose on NF-κB binding activity in HAECs. (C) Dose-dependent effect of iron sucrose on expression of VCAM-1 and ICAM-1 in HAECs. Effect of NF-κB p65 siRNA on (D) VCAM-1/ICAM-1 expression and (E) U937 adhesion in iron-stimulated HAECs and (F) antagonist effect of anti–VCAM-1 (10 μg/ml) antibody or anti–ICAM-1 (10 μg/ml) antibody on U937 adhesion in iron-treated HAECs. Values are expressed as means±SEMs of five separate experiments. *P<0.05 compared with baseline; ^#P<0.05 compared with iron-treated groups.