Figure 4.
Intraperitoneal iron enhanced superoxide production and upregulated the expression of adhesion molecules in SNx wild-type mice. (A) Representative images of superoxide production in iron-loaded sham or SNx mice. (B) Quantitative data of CL counts in iron-loaded sham or SNx mice; the data were presented as CL counts per 10 s/μg protein from aortic tissue lysates. Expression of (C) VCAM-1 and (D) ICAM-1 in aortic tissue lysates in iron-loaded sham or SNx mice. (E) Representative images of immunohistochemical staining of p22phox in aorta of iron-loaded sham or SNx mice. The arrows indicate positive signals of p22phox in endothelium, and the arrowheads indicate positive signals of p22phox in the media layer. Data are expressed as means±SEMs. Number of animals examined in each group was 10. Scale bar, 20 μm.