Figure 1.
Dicer-1 depletion in mCCD cells reduces aldosterone stimulation of ENaC-mediated Na+ transport. (A) Western blot of whole-cell lysates collected from control siRNA and Dicer-1 knockdown mCCD cells grown on filter supports. (B) Representative short-circuit current (ISC) traces from mCCD cells mounted in modified Ussing chambers. Control and Dicer siRNA transfected cells stimulated with 10 nM aldosterone for 24 hours before ISC measurements. (C) Summarized data (mean±SEM) from similar experiments (n>12) to those presented in B. cont, control.