Figure 3.

Expression of miRs is decreased in distal nephron epithelial cells isolated from mice receiving low-sodium diets. qRT-PCR miR expression in CCD cells isolated from kidneys of mice receiving low-sodium diets (black bars) or from cultured CCD cells stimulated with aldosterone (gray bars) was normalized to control expression. The fold change in miR expression did not significantly differ between in vivo and in vitro CCD cells. Expression of miR-1983, miR-335–3p, and miR-290–5p was significantly reduced from mice receiving normal diets or unstimulated mCCD cells. As a control, no difference in expression was observed for miR-10a after low-sodium diet or aldosterone stimulation (in agreement with the microarray data, see Supplemental Figure 1).