Figure 5.

Exogenous increase in miR expression reduces aldosterone-stimulated ENaC transport. (A) Normalized I_SC measurements from mCCD were transiently transfected with plasmids encoding pre-miR sequences, alone or in combination. Cells seeded onto filter supports were stimulated with aldosterone for 24 hours (50 nM), and I_SC was normalized to unstimulated mCCD cells transfected with control plasmid. I_SC response to aldosterone decreased significantly (*P<0.05) in cells overexpressing miR-1983, miR-290–5p, and miR-335–3p or the combination of the three miRs (n=30). (B) The same experiments as in A were carried out using miR mimics, and a significant reduction in aldosterone response was seen for all overexpressed miRs (n=10).