Figure 1.

Mafb overexpression was observed in podocyte-specific Mafb transgenic kidneys. (A) Schematic of the Nephrin promoter-Mafb transgene construct. The Mafb cDNA was inserted into a vector that contained the human Nephrin promoter (Nep) and SV40 polyA (pA). The probe for Southern blot analysis, the restriction enzyme site (EcoRI [E]), and the predicted sizes of the endogenous gene and the transgene are indicated. (B) The 5.0-kb endogenous and 1.5-kb transgene fragments are shown from TG mice. (C) Analysis of Mafb mRNA in kidneys. The amount of Mafb mRNA in the samples from TG mice (TG-1 and TG-2) was higher than that in a sample from WT mice. Each bar represents the mean±SEM. **P<0.01. (D) Western blot analysis of kidneys from WT and Mafb TG mice (TG-1 and TG-2). Expression of Mafb protein was elevated in TG mice. Hprt, hypoxanthine-guanine phosphoribosyltransferase.