Overexpression of Mafb partially restores podocyte number. (A) Typical images of WT1-stained glomeruli from WT CON, TG CON, WT STZ, and TG STZ mice. (B) Average number of WT1-stained nuclei calculated per glomerular sections. The number of WT1-stained nuclei was obtained from 10 glomerular sections per kidney sample. Values represent the means±SEMs (n=4 mice per group). *P<0.05. (C) Diabetes-induced glomerular cell death is reduced by Mafb overexpression. TUNEL staining in WT CON, TG CON, WT STZ, and TG STZ glomeruli. Quantification of TUNEL staining in all glomerular sections per kidney sample. Values represent the means±SEMs (n=4 mice per group). *P<0.05; **P<0.01. (D) Apoptotic glomerular cells were podocytes in diabetic glomeruli. TUNEL-positive apoptotic podocyte in the glomeruli of WT STZ mice was WT1-positive (arrows). Blue, 4′,6-diamidino-2-phenylindole (DAPI); green, TUNEL; red, WT1. Scale bar, 50 μm.