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. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: J Allergy Clin Immunol. 2009 Jan 18;123(4):933–9.e10. doi: 10.1016/j.jaci.2008.11.037

FIG 2.

FIG 2

CCR4 and CCR8 expression by Treg cells. A, Gating of Treg cells from PBMCs. Lymphocytes were gated by means of characteristic side scatter and forward scatter. CD4+ T cells were gated from these lymphocytes. From CD4+ T cells, Treg cells were identified by using CD127 and CD25 markers. CD25 and CD127 gates were defined by using isotype controls. PerCP, Peridinin-chlorophyll-protein complex; FITC, fluorescein isothiocyanate; PE, phycoerythrin. B, CCR4 and CCR8 expression by Treg cells. CCR4 and CCR8 expression by Treg cells was measured based on mean fluorescence intensity (MFI), as well as percentages of cells that express these chemokine receptors. ANOVA was used for all analyses because all data sets have normal distribution and equal variances.

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