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. 2014 Oct 30;10(10):e1004707. doi: 10.1371/journal.pgen.1004707

Figure 1. Starvation downregulates the expression of srh-234 in ADL neurons.

Figure 1

A) Expression of srh-234p::gfp in an ADL neuron of wild-type animals when well-fed in the presence of E. coli OP50 food (upper panel) and starved (lower panel) in the absence of food for 12 hours. The head of the animal is indicated with a white line. Arrow points to the ADL cell body. Images were acquired at the same exposure time. Lateral view: anterior is on the left. Scale, 100 µm. B) Levels of endogenous srh-234 messages are downregulated in starved animals compared to fed animals. Shown is the ratio of endogenous srh-234 message to endogenous odr-10 [65] message as quantified by qRT–PCR in fed and starved animals. In hermaphrodite animals, expression of odr-10 is unaffected by starvation. The mean of the ratios from two independent experiments is shown. * indicates values that are different from fed wild-type animals at P<0.01 using a two-sample t-test. Error bars denote the SEM.