Figure 3. daf-2 and daf-16a are required in ADL, but not in the intestine, to regulate srh-234 expression.

A) Percentage of mutant animals with defects in insulin signaling expressing srh-234p::gfp at wild-type levels. daf-2(e1307) is a temperature sensitive allele. In all experiments, animals were raised at 15°C (permissive temperature) and shifted to the 25°C (restrictive temperature) as L4 larvae. Animals (n>150) were examined at 150× magnification for each genotype. B, C) Relative expression of srh-234p::gfp in daf-2 or daf-16 mutants carrying compared to wild-type when fed or starved. For strains carrying ADL::daf-2 cDNA, pan-neural::daf-2 cDNA, intestine::daf-2 cDNA, ADL::daf-16a cDNA and intestine::daf-16a cDNA extrachromosomal arrays (see Material and Methods), data shown is for at least two independent transgenic lines. Animals (n = 18–25) were examined at 400× magnification for each genotype. * indicates values that are different from that of wild-type animals at P<0.001, # and ## indicates the values that are different between the genotypes compared by brackets at P<0.001, and P<0.05, respectively, using either a two-sample t-test or a χ² test of independence. Error bars denote the SEM or SEP.