Table 1. WNV isolates used in this study.

	Isolate	Location	Year	Species	Genbank accession	E-159	NS4A-85
East Coast genotype
1	NY99	New York	1999	Chilean flamingo	AF196835	V	A
2	NY2001	New York	2001	Human	KJ786934	V	A
WN02 genotype
3	TX114	Texas	2002	Blue jay	GU827998	A	A
4	TX7191	Texas	2007	Blue jay	JF415920	A	A
5	TXAR7465	Texas	2009	Culex tarsalis	JX015521	A	A
6	TX8759	Texas	2012	Blue jay	KJ786936	A	A
7	TX8779	Texas	2012	Northern mockingbird	KJ786935	A	A
8	TX2600	Texas	2012	Cx. quinquefasciatus	KC736487	A	A
SW03 genotype
9	M19433	Texas	2009	Aedes albopictus	JF415919	A	T
10	TXAR6115	Juarez	2009	Cx. quinquefasciatus	JX015520	A	T
11	TXAR6572	Texas	2010	Cx. tarsalis	JX015523	A	T
12	TX2689	Texas	2012	Cx. quinquefasciatus	KC736492	A	T