Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1977 Mar;15(3):945–949. doi: 10.1128/iai.15.3.945-949.1977

Transfection of Streptococcus sanguis by phage deoxyribonucleic acid isolated from Streptococcus mutans.

M Higuchi, G H Rhee, S Araya, M Higuchi
PMCID: PMC421464  PMID: 858646

Abstract

Streptococcus sanguis ATCC 10556 cells were infected with free phage DNA of S, mutans strain PK 1. Two transformants were isolated which made colonies with large mucoid forms on mitis-salivarius agar. Both transformants had an increased ability to synthesize insoluble glucan and showed an adhesive nature on glass surfaces. These characteristics of the transformants bear a resemblance to S. mutans. These transformants had many physiological characteristics by which they could be recognized as S. sanguis. However, they resembled S. salivarius in forming a large amount of soluble fructan. Furthermore, the transformant cells did not produce ammonia from arginine, whereas their parent cells did.

Full text

PDF
945

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  2. Bratthall D. Immunofluorescent identification of Streptococcus mutans. Odontol Revy. 1972;23(2):181–196. [PubMed] [Google Scholar]
  3. Brown A. T., Patterson C. E. Heterogeneity of Streptococcus mutans strains based on their mannitol-1-phosphate dehydrogenases: criterion for rapid classification. Infect Immun. 1972 Sep;6(3):422–424. doi: 10.1128/iai.6.3.422-424.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown A. T., Wittenberger C. L. Fructose-1,6-diphosphate-dependent lactate dehydrogenase from a cariogenic streptococcus: purification and regulatory properties. J Bacteriol. 1972 May;110(2):604–615. doi: 10.1128/jb.110.2.604-615.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carlsson J. A numerical taxonomic study of human oral streptococci. Odontol Revy. 1968;19(2):137–160. [PubMed] [Google Scholar]
  6. Coykendall A. L. Base composition of deoxyribonucleic acid isolated from cariogenic streptococci. Arch Oral Biol. 1970 Apr;15(4):365–368. doi: 10.1016/0003-9969(70)90063-4. [DOI] [PubMed] [Google Scholar]
  7. Coykendall A. L. Genetic heterogeneity in Streptococcus mutans. J Bacteriol. 1971 Apr;106(1):192–196. doi: 10.1128/jb.106.1.192-196.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DISCHE Z., DEVI A. A new colorimetric method for the determination of ketohexoses in presence of aldoses, ketoheptoses and ketopentoses. Biochim Biophys Acta. 1960 Mar 25;39:140–144. doi: 10.1016/0006-3002(60)90129-3. [DOI] [PubMed] [Google Scholar]
  9. Dunny G. M., Hausner T., Clewell D. B. Buoyant densities of DNA from various strains of Streptococcus mutans. Arch Oral Biol. 1972 Jun;17(6):1001–1003. doi: 10.1016/0003-9969(72)90123-9. [DOI] [PubMed] [Google Scholar]
  10. Edwardsson S. Characteristics of caries-inducing human streptococci resembling Streptococcus mutans. Arch Oral Biol. 1968 Jun;13(6):637–646. doi: 10.1016/0003-9969(68)90142-8. [DOI] [PubMed] [Google Scholar]
  11. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  12. Greer S. B., Hsiang W., Musil G., Zinner D. D. Viruses of cariogenic streptococci. J Dent Res. 1971 Nov-Dec;50(6):1594–1604. doi: 10.1177/00220345710500064101. [DOI] [PubMed] [Google Scholar]
  13. Higuchi M., Araya S., Higuchi M. Plasmid DNA satellite bands seen in lysates of Streptococcus mutans that form insoluble extracellular polysaccharides. J Dent Res. 1976 Mar-Apr;55(2):266–271. doi: 10.1177/00220345760550021801. [DOI] [PubMed] [Google Scholar]
  14. Higuchi M., Endo K., Hoshino E., Araya S. Preferential induction of rough variants in Streptococcus mutans by ethidium bromide. J Dent Res. 1973 Sep-Oct;52(5):1070–1075. doi: 10.1177/00220345730520051401. [DOI] [PubMed] [Google Scholar]
  15. Higuchi M., Rhee G. H., Araya S., Higuchi M. Bacteriophage deoxyribonucleic acid-induced mutation of Streptococcus mutans. Infect Immun. 1977 Mar;15(3):938–944. doi: 10.1128/iai.15.3.938-944.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hojo S., Huguchi M., Araya S. Glucan inhibition of diffusion in plaque. J Dent Res. 1976 Jan-Feb;55(1):169–169. doi: 10.1177/00220345760550011501. [DOI] [PubMed] [Google Scholar]
  17. Klein J. P., Frank R. M. Mise en évidence de virus dans les bactéries cariogénes de la plaque dentaire. J Biol Buccale. 1973 Mar;1(1):79–85. [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  20. Scherp H. W. Dental caries: prospects for prevention. Science. 1971 Sep 24;173(4003):1199–1205. doi: 10.1126/science.173.4003.1199. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES