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. 2014 Oct 30;9(10):e110758. doi: 10.1371/journal.pone.0110758

Table 3. Composition of the experimental and chow diets.

HFD1 HFD + Plant sterol esters HFD + Plant stanol esters Chow
Composition (%)
Sucrose 39.75 38.97 38.97 29.38
Casein 23.64 23.18 23.18 20.00
Beef fat 15.78 15.47 15.47 -
Cellulose 5.91 5.79 5.79 5.00
Olive Oil4 2.94 2.07 2.07 2.00
Soybean oil4 2.27 2.07 2.07 2.00
Corn Starch 2.59 2.54 2.54 35.92
Vitamin Mix2 0.58 0.58 0.58 0.50
Mineral Mix3 5.44 5.33 5.33 4.60
Choline 0.47 0.46 0.46 0.40
DL Methionine 0.24 0.23 0.23 0.20
Cholesterol4 0.20 0.20 0.20 -
Linseed Oil5 0.19 - - -
Plant sterol esters6 - 3.10 - -
Plant stanol esters6 - - 3.10 -
1

HFD: high fat diet;

2

Vitamin mix: vitamins premix, trace elements premix;

3

Mineral mix: calcium hydrogen phosphate, calcium carbonate, potassium chloride, potassium dihydrogen phosphate, magnesium sulphate heptahydrate, sodium chloride, magnesium oxide;

4

This added amount of 0.2% cholesterol together with the 0.015% cholesterol from beef fat makes that the diet contains 0.22% cholesterol;

5

The small amounts of olive oil, soybean oil and linseed oil were added to the HFD and not to the HFD + sterol or stanol esters to make the amount and type of fatty acids in the three HF diets comparable since the fatty acids in the sterol and stanol esters (rapeseed oil fatty acids) become available during digestion.

6

The 3.1% plant sterol or stanol esters correspond to ±2% free plant sterols or stanols. The plant stanols used are a mixture of mainly sitostanol and campestanol 85/15 and the plant sterols used are a mixture of mainly sitosterol and campesterol 70/30.

The chow diet contains ±10.2 en% fat, whereas the HFD contains ±41.5 en% fat.