Figure 12. SubAB failed to block thrombin-induced IκBα degradation.

HPAEC were treated with 0.1 µg/ml of SubAB or mutant SubA_A272B for 6 h, followed by treatment with ( A ) thrombin (5 U/ml) for 1 h or with ( C ) TNFα (100 U/ml) for 30 min. Total cell lysates were prepared and immunoblotted with anti-IκBα antibody to determine degradation of IκBα. Levels of RelA/p65 was used to monitor loading. The bar graphs represent the effect SubAB and SuBA_A272B on ( B ) thrombin-induced or ( D ) TNFα-induced IκBα degradation normalized to total RelA/p65 levels. The data are the means ± S.E. (n = 3–6 for each condition). ^# p<0.05 difference from controls.