Skip to main content
. 2014 Oct 30;9(10):e110949. doi: 10.1371/journal.pone.0110949

Figure 13. SubAB attenuates RelA/p65 DNA binding independent of its nuclear translocation.

Figure 13

HPAEC were treated with 0.1 µg/ml of SubAB or mutant SubAA272B for 6 h, followed by treatment with ( A & C ) thrombin (5 U/ml) for 1 h or with ( B & D ) TNFα (100 U/ml) for 30 min. ( A & B ) Nuclear extracts (NE) were separated by SDS-PAGE and immunoblotted with anti-RelA/p65 antibody. Tata Binding Protein (TBP) was used as a loading control for nuclear extract. ( C & D ) Nuclear extracts were assayed for DNA binding of RelA/p65 by Cayman’s NF-κB (RelA/p65) Transcription Factor Assay Kit as described in Materials and Methods. The data are the means ± S.E. (n = 6 for each condition). ### p<0.001 difference from controls; *p<0.05 difference from thrombin and TNF-α stimulated controls.