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. 2014 Oct 30;9(10):e110834. doi: 10.1371/journal.pone.0110834

Figure 2. Purification and characterization of LacA from Cerrena sp. HYB07.

Figure 2

(A) Time course of laccase activity and secreted protein levels produced by Cerrena sp. HYB07. Error bars represent standard deviations of triplicate experiments. (B) SDS-PAGE analysis of LacA. Lane M1, Protein molecular weight marker (Low) by Takara; lane 1, purified LacA; lane 2, purified LacA after peptide N-glycosidase F treatment; lane M2, Blue Plus II Protein Marker (Transgen, China); lane 3, purified LacA. Lanes 1 and 2 were stained with Coomassie Brilliant Blue R-250 after reducing electrophoresis; lane 3 was stained with guaiacol after non-reducing electrophoresis, and the sample was not heated before loading. (C) UV-Vis absorbance spectrum of purified LacA.