Table 1. Primers used in this study.
| Primer | Oligonucleotide sequence | Reference |
| NS1 | 5′-GTAGTCATATGCTTGTCTC-3′ | [22] a |
| NS8 | 5′-TCCGCAGGTTCACCTACGGA-3′ | |
| Cu I | 5′-CAYTGGCAYGGNTTYTTYCA-3′ | [29] b |
| Cu IV | 5′-TGVHARTCDATRTGRCARTG-3′ | |
| LacA SP1 | 5′- CGACATCATAGAGAGCCTTGTGCGGA -3′ | This studyc |
| LacA SP2 | 5′- TCAGTGGGATCGTAGACAACAAAGGCA -3′ | |
| LacA SP3 | 5′- AGATGGGGCACTGGTTCACGA -3′ | |
| LacA SP4 | 5′- TCTGGCTCCGTCATTACCCTTCCCATC -3′ | |
| LacA SP5 | 5′- AGACCATTGAACTTACCCTTGCAGCG -3′ | |
| LacA SP6 | 5′- GGTCAAACTACTCCCAACTACG -3′ | |
| AD1 | 5′- TGWGNAGWANCASAGA-3′ | [30] d |
| AD2 | 5′-AGWGNAGWANCAWAGG-3′ | |
| AD3 | 5′-STTGNTASTNCTNTGC-3′ | |
| AD4 | 5′-NTCGASTWTSGWGTT -3′ | |
| AD5 | 5′-NGTCGASWGANAWGAA -3′ | |
| AD6 | 5′-WGTGNAGWANCANAGA -3′ | |
| AD7 | 5′-WTCTGNCTWANTANCT-3′ | |
| LacA GSP1 | 5′-GGTAGCGATGCCGAATGAGGG-3′ | This studye |
| LacA GSP2 | 5′- AGATGGGGCACTGGTTCACGA -3′ | |
| SFP1 | 5′-CACGACACGCTACTCAACACACCA-3′ | [31] f |
| SFP2 | 5′-AACACACCACCTCGCACAGC-3′ | |
| SiteFinder1 | 5′-CACGACACGCTACTCAACACACCACCTCGCACAGCGTCCAAGCGGCCGCNNNNNNGCCT-3′ | |
| SiteFinder2 | 5′-CACGACACGCTACTCAACACACCACCTCGCACAGCGTCCAAGCGGCCGCNNNNNNGCGC-3′ | |
| LacA-RT-1 | 5′- ATGGCATTCCGTTCAGGC -3′ | This studyg |
| LacA-RT-2 | 5′- TTACTTGTCACCATCAGCAAGA -3′ | |
| R = A/G, Y = C/T, M = A/C, K = G/T, S = G/C, W = A/T, H = A/T/C, D = G/A/T, B = G/T/C, V = G/A/C, N = A/T/G/C. |
a
Universal primers for amplification of 18S rDNA.
b
Degenerate primers for amplification of the laccase sequence spanning the first and the fourth copper-binding motifs.
c
Gene-specific primers for TAIL-PCR to amplify the 5′- and 3′-flanking sequences of the laccase fragment obtained with primers Cu I and Cu IV.
d
Arbitrary degenerate primers for TAIL-PCR.
e
Gene-specific primers for SiteFinding PCR to amplify the LacA promoter sequence.
f
SiteFinders and their primers (SFP1 and SFP2) for SiteFinding PCR.
g
Primers for amplification of the cDNA sequence of LacA.