Table 3. Effect of inhibitors and metal ions on LacA activity.
Inhibitor | Concentration (mM) | Relative activity (%) | Metal ion | Concentration (mM) | Relative activity (%) |
L-Cysteine | 0.01 | 87.5±1.7 | Na+ | 10 | 115.1±0.9 |
0.1 | 5.5±0.1 | K+ | 10 | 109.8±1.7 | |
DTT | 0.01 | 38.4±7.1 | Li+ | 10 | 48.8±0.9 |
0.1 | 5.9±0.7 | Cu2+ | 10 | 101.9±4.3 | |
EDTA | 1 | 95.4±3.2 | Ca2+ | 10 | 113.6±2.0 |
10 | 82.4±2.4 | Mg2+ | 10 | 111.6±3.2 | |
NaN3 | 0.01 | 30.2±2.5 | Mn2+ | 10 | 117.3±2.3 |
0.1 | 9.2±1.5 | Zn2+ | 10 | 111.0±3.9 | |
SDS | 0.01 | 80.8±0.9 | Fe2+ | 10 | 3.8±0.8 |
0.1 | 11.4±1.2 | Cr2+ | 10 | 99.0±1.1 | |
Kojic acid | 12.5 | 49.3±1.8 | Co2+ | 10 | 99.8±4.1 |
50 | 31.7±3.2 | Pb2+ | 10 | 93.8±3.7 |
Values represent mean ± standard deviation (n = 3).