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. 2014 Oct 30;9(10):e111354. doi: 10.1371/journal.pone.0111354

Figure 2. Expression pattern of the BnNF-Y genes in plants subjected to drought stress.

Figure 2

The expression of BnNF-YA (A), BnNF-YB (B), and BnNF-YC (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.