Table 1. Primers information and RT-qPCR conditions used in the trial.

Gene1	NCBI accession number	Oligo sequence (5′→3′)		Amplicon length	Annealing T
ATP4A	M22724	Forward Reverse	GCATATGAGAAGGCCGAGAG TGGCCGTGAAGTAGTCAGTG	151 pb	57°C
GAST	NM_001004036	Forward Reverse	GACTCTGCGCCTATGTCCTG GCTCTTTGCCCCTGTTGG	133 bp	60°C
GHRL	NM213807	Forward Reverse	GAACAGAGGTGGCTGGTCTC ACAGGGGAGACAAGGAAAGG	202 pb	62°C
PIGR	NM_214159.1	Forward Reverse	AGCCAACCTCACCAACTTCC CTGCTAATGCCCAGACCAC	105 bp	62°C
HMBS	DQ845174	Forward Reverse	AGGATGGGCAACTCTACCTG GATGGTGGCCTGCATAGTCT	83 bp	62°C
RPL4	DQ845176	Forward Reverse	CAAGAGTAACTACAACCTTC GAACTCTACGATGAATCTTC	122 bp	60°C

¹ ATP4A, H+/K+ Atpase α; GAST, gastrin; GHRL, ghrelin/obestatin prepropeptide; PIGR, polymeric immunoglobulin receptor; HMBS, hydroxymethylbilane synthase; RPL4, ribosomal protein L4.