Table 2. Substrate specificity of fusolisin.
| A | |
| Fibrinogen | Peptide Intensity |
| GTAWT/A | 4.5×109 |
| SGSSG/P | 9.0×108 |
| TAWTA/D | 4.0×108 |
| LGGWL/L | 3.8×108 |
| NFNRT/W | 3.8×108 |
| AWTAD/S | 1.6×108 |
| PRNPS/S | 2.6×107 |
| B | |
| FRETS-25 Thr | Peptide Intensity |
| T/AFPKRR | 3.6×108 |
| GFIT/A | 8.6×107 |
| GVIT/A | 4×107 |
| GEIT/A | 2.4×107 |
| GFPT/A | 1.6×107 |
A) Major peptides obtained by fusolisin hydrolysis of fibrinogen.
B) Major peptides obtained by fusolisin hydrolysis of FRETS-25 Thr.
D-A2pr(Nma)- Gly- [Phe/Ala/Val/Glu/Arg] - [Pro/Tyr/Lys/Ile/Asp]- Thr- Ala- Phe- Pro- Lys(Dnp)- D-Arg- D-Arg TRIFLUOROACETATE.
Mass spectrometry was performed as described in Materials and Methods. The peptide intensity based on the peak area was analyzed by LC-MS and ordered by decreasing abundance.