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. 2014 Nov 1;25(21):3319–3329. doi: 10.1091/mbc.E14-06-1132

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© 2014 Kim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Kif18A-tail chimeras rapidly accumulate at K-fiber ends in Taxol-treated cells. (A) Fluorescence micrographs of the indicated GFP-tagged kinesins (green) in Kif18A-depleted HeLa cells after a 5-min incubation with 10 μM Taxol. Cells were immunostained with ACA (centromeres, red) and tubulin (blue). (B) Line scans of GFP-tagged kinesins (green trace) relative to tubulin (blue trace) and ACA (red trace) along K-fibers in 10 μM Taxol–treated cells. (C) Still images from live-cell analyses of GFP-tagged kinesin relocalization after the addition of Taxol. Taxol was added immediately after the 0-s image was captured.