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. 2014 Nov 1;25(21):3319–3329. doi: 10.1091/mbc.E14-06-1132

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© 2014 Kim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Kif18A's loop2 is necessary but not sufficient for motor accumulation at K-fiber ends. (A) Protein sequence alignment of the loop2 regions for the indicated kinesins. Kif18A's loop2 contains six lysine residues (underlined), which were mutated to alanine (orange, underlined) in Kif18A-L2-K6A. (B) Western blots of lysates transfected with the indicated siRNAs and GFP-tagged transgenes. Purified Kif18A-GFP (30 ng) was also loaded for comparison. Blots were probed with anti-Kif18A (top) and anti-GAPDH (bottom) antibodies. (C) Fluorescence micrographs of the indicated GFP-tagged kinesins (green) in cells immunostained with ACA (centromeres, red) and tubulin (blue). (D) Line scans of GFP-tagged kinesin (green trace) relative to tubulin (blue trace) and ACA fluorescence (red trace) along peripheral K-fibers.