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. 2014 Nov 1;25(21):3319–3329. doi: 10.1091/mbc.E14-06-1132

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© 2014 Kim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Kif18A's loop2 is necessary but not sufficient for stable association with K-fiber ends. (A) Fluorescence micrographs of the indicated GFP-tagged loop2 mutant kinesins (green) in Kif18A-depleted HeLa cells after a 5-min incubation with Taxol. Cells were immunostained with ACA (centromeres, red) and tubulin (blue). (B) Line scans of GFP-tagged loop2 mutants (green trace) relative to tubulin (blue trace) and ACA (red trace) along K-fibers in 10 μM Taxol–treated cells. (C) Still images from live-cell analyses of GFP-tagged kinesin relocalization after the addition of 10 μM Taxol. Taxol was added immediately after the 0-s image was captured. (D, E) Plots displaying relative GFP fluorescence as a function of time after a bleaching event for the indicated kinesins. Dashed lines are single-exponential fits to the data. Quantified data from FRAP experiments are reported in Table 1.