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. 2014 Nov 1;25(21):3319–3329. doi: 10.1091/mbc.E14-06-1132

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© 2014 Kim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Kinesin-8 loop2 is required for mitotic chromosome alignment and spindle length regulation. (A) Schematic of experimental design. Representative images show control and Kif18A siRNA–treated cells immunostained with ACA (centromeres, green) and γ-tubulin (red) antibodies. (B) Representative plot of centromere (ACA) fluorescence distribution as a function of position along the pole-to-pole axis. Fluorescence data were fitted with a Gaussian function, and the full-width at half-maximum (FWHM) was calculated as a metric for centromere alignment. (C, D) Graphs of average FWHM (C) and average spindle length (D) calculated from cells transfected with the indicated siRNAs and transgenes. **p < 0.01.