TABLE 1:
FRAP measurements of kinesin behavior at K-fiber ends in Taxol-treated cells.
| GFP-kinesin | t1/2 (s) | Mobile fraction (%) | Fraction recovered 30 s after bleach (%) | Number of cells |
|---|---|---|---|---|
| Kif18A-FL | 45.0 ± 20.0 | 18.6 ± 8.8 | 6.6 ± 4.0 | 8 |
| Kif18B-18A | 19.5 ± 5.0** | 20.9 ± 8.2 | 12.2 ± 4.9 | 4 |
| Kif4A-18A | 10.4 ± 3.3** | 43.2 ± 21.5** | 40.4 ± 20.7** | 9 |
| Kif5B-18A | 8.3 ± 2.9** | 57.5 ± 16.3** | 51.9 ± 14.3** | 6 |
| Kif18A-770 | 20.4 ± 8.0** | 39.1 ± 17.0** | 23.5 ± 10.5** | 9 |
| Kif18A-L2-K6A | 16.2 ± 5.7** | 37.6 ± 17.0** | 29.4 ± 14.9** | 10 |
| Kif18A-ΔL2 | 13.7 ± 8.4** | 30.8 ± 10.7* | 22.9 ± 9.9** | 6 |
| Kif4A-L2-18A | 18.7 ± 9.9**,# | 25.9 ± 5.0*,# | 16.9 ± 6.1**,# | 9 |
*p ≤ 0.05 compared with Kif18A-FL, **p ≤ 0.01 compared with Kif18A-FL, #p ≤ 0.05 compared with Kif4A-18A.