Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 1;25(21):3342–3349. doi: 10.1091/mbc.E14-07-1178

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Heublein et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — Kgd4 copurifies with α-KGDH. (A) Strategy to analyze the composition of KGDH. (B) Mitochondrial KGDH was purified using a C-terminal His7-tag on Kgd1. Proteins were separated on SDS–PAGE and analyzed by Coomassie staining and mass spectrometry. Boxes indicate proteins specifically identified in the elution fraction of the sample containing His7-tagged Kgd1. (C) Complexes of Ymr31 were purified as in B, and the purifications were analyzed by Western blotting using the indicated antibodies. T, total of the lysate; NB, unbound fraction; W, wash fraction; E, elution fraction.