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. 2014 Nov 1;25(21):3342–3349. doi: 10.1091/mbc.E14-07-1178

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© 2014 Heublein et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Kgd4 function is evolutionarily conserved. (A) Alignment of amino acid sequences of Kgd4 homologues. Sequences used for comparison were extracted from the National Center for Biotechnology Information database (www.ncbi.nlm.nih.gov/protein): S. cerevisiae (Sc-gi45270840); Neurospora crassa (Nc-gi85092531), Schizosaccharomyces pombe (Sp-gi429240132), Podospora anserina (Pd-gi171678223), Homo sapiens (Hs-gi15150811), Rattus norvegicus (Rn-gi300797955), Mus musculus (Mm-gi13384742), and Bos taurus (Bt-gi78369264). (B) Phylogenetic tree of Kgd4 homologues from fungi and animals. Sequences used for comparison were extracted as in Figure 4A. (C) siRNA-mediated knockdown of KGD4 in murine glial cells. (D) Quantification of the knockdown efficiency. (E) Cells transfected with control RNA were lysed and subjected to density gradient centrifugation. The fractions were analyzed by Western blotting. (F) Cells in which KGD4 was depleted for 48 h were processed and analyzed as in D.