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. 2014 Nov 1;25(21):3389–3400. doi: 10.1091/mbc.E14-06-1087

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© 2014 Ling et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Osh4 regulates PI4P distribution and dynamics in cells. (A) Localization of the PI4P probe GFP-2xPH^Osh2 in wild-type, osh4Δ, sac1Δ, and inp52Δ inp53Δ cells. Cells shown are representative of >100 cells observed. Scale bar, 5 μm. (B) Localization of the PI4P probe GFP-2xPH^Osh2 and mCherry-Sec4 in wild-type and osh4Δ cells. Cells shown are representative of >100 cells observed. Scale bar, 5 μm. (C) PI4P dynamics in wild-type and osh4Δ cells. Cells expressing GFP-2xPH^Osh2 were examined by time-lapse fluorescence microscopy. Images are shown at indicated time points. Arrows indicate that GFP-2xPH^Osh2 accumulated at polarized growth sites. Scale bar, 2 μm.