Figure 2. Synthetic septation phenotypes observed in double mutants in genes encoding ESCRT proteins and (a) plo1-ts35, (b) ark1-T8 or (c) clp1Δ.

Fission yeast double mutant strains were grown in complete liquid medium at 25°C to mid-exponential phase and harvested. Cells were stained with Calcofluor white and visualised using fluorescence microscopy. Both fluorescence and bright field images are shown. Scale bar, 10 µm. The frequency of phenotypes A–F (described in Figure 1a) was quantitatively analysed in strains containing double mutants, in comparison to each parent. In each case 400 cells were counted in triplicate (*p<0.05). Each of the ESCRT genes labels is accompanied by its respective ESCRT complex identification (E-0, E-I, E-II and E-III).