Figure 7. Genetic interactions between plo1-ts35, ark1-T8 and clp1Δ, and ESCRT deletions in controlling vacuolar cell sorting in fission yeast.

Double mutants of ESCRT deletions and (a) plo1-ts35, (b) ark1-T8 or (c) clp1Δ, transformed with Ub-GFP-SpCPS, were cultured in liquid minimal medium at 25°C, stained with FM 4-64, and visualised using confocal microscopy. Scale bar, 10 µm.