Figure 8. Deletion mutants with defects in salivary gland infection rates are SoMo-positive.
A and B. MKK1 and PSSA-2 deletion mutants were cultured with or without glycerol. 4×105 cells from each culture were inoculated onto plates containing 20 mM glycerol. The scale bar is 1 cm. Four days post plating community lifts were incubated with α-EP and α-GPEET antibodies. C. 2×105 cells of the procyclin null mutant (Δproc), obtained by differentiation of bloodstream forms, were inoculated onto a 0.4% agarose plate containing 20 mM glycerol. A photograph was taken 4 days post plating.