Table 3. Adipose concentration of PAI-1 and plasma concentrations of adipokines (PAI-1, MCP-1, TNF-α, leptin) and uPA¹.

	Treatment					p value
	Control	AIN WT	AIN PAI-1−/−	HF WT	HF PAI-1−/−	Diet	Gene	D × G
PAI-1,2 ng/mg protein	0.22±0.03	0.17±0.04b	N.D.	0.42±0.07a	N.D.	<0.01	<0.01	<0.01
PAI-1, ng/mL	0.44±0.10*	2.23±0.30b	N.D.	4.50±0.48a	N.D.	<0.01	<0.01	<0.01
uPA, ng/mL	0.82±0.06*	1.76±0.20	1.51±0.23	3.77±0.35	3.15±0.33	<0.01	0.14	0.51
MCP-1, pg/mL	7.32±1.97*	39.77±4.85b	43.21±2.27b	87.77±3.65a	114.88±8.37a	<0.01	<0.01	<0.05
TNF-α, pg/mL	1.93±0.64*	9.60±0.30	10.53±0.32	16.51±0.39	17.49±0.47	<0.01	<0.05	0.94
Leptin, ng/mL	4.53±0.42	4.57±0.35	6.48±0.60	10.18±0.75	12.53±1.14	<0.01	0.01	0.77

¹Two-way ANOVA and Tukey contrasts were performed to compare differences among the groups of LLC-bearing mice; a priori contrasts were performed to compare differences in AIN93G-fed wild-type mice with or without LLC (control vs. AIN WT). Values (means ± SEM) in a row with different superscripts are significantly different at p≤0.05 for LLC-bearing groups (n = 10 per group).

*p<0.01 compared to AIN WT. Control: AIN93G-fed non-tumor-bearing wild-type mice, AIN WT: AIN93G-fed wild-type mice, AIN PAI-1^−/−: AIN93G-fed PAI-1^−/− mice, HF WT: high-fat fed wild-type mice, HF PAI-1^−/−: high-fat fed PAI-1^−/− mice, D × G: diet × gene interaction, N.D.: not detectable.

²Adipose PAI-1.