Fig. 2.

A: representative Western blot analysis of cyclooxygenases (COXs) in the lysates of MC38 cells. Western blot analysis showed that COX-1 and COX-2 are highly expressed in MC38 cells. Based on protein standards, the size of COXs is ∼72 kDa. B: PGE₂ levels were higher in media than in cell lysates of MC38 cells. C: effect of rofecoxib on PGE₂ production in MC38 cells, and results are expressed as %control. PGE₂ production was estimated by a PGE₂ ELISA. Values are means ± SE; n = 5. **P < 0.01 vs. cell lysates. ##P < 0.01 vs. other groups.